C9891

Sigma

 

Collagenase from Clostridium histolyticum

Type IA, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid (CDU = collagen digestion units), essentially salt-free, lyophilized powder

Synonym:Clostridiopeptidase A
CAS Number:9001-12-1
Enzyme Commission (EC) Number:3.4.24.3   ( BRENDA | IUBMB )
EC Number:232-582-9
MDL number:MFCD00130830

Description

Analysis NoteAlso contains clostripain, nonspecific neutral protease, and tryptic activities.
LinkageSimilar to Type I, but produced by Sigma.
Other NotesView more information on collagenase and cell dissociation at www.sigma-aldrich.com/enzymeexplorer.
Preparation NoteEquivalent to first 40% ammonium sulfate fraction of Mandl, I., et al., J. Clin. Invest., 32, 1323 (1953).
Unit DefinitionOne collagen digestion unit liberates peptides from collagen equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hr at pH 7.4 at 37 °C in the presence of calcium ions.
One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions.
One neutral protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine per 5 hr at pH 7.5 at 37 °C.
One clostripain unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of DTT.

Properties

descriptionFor general use
formessentially salt-free, lyophilized powder
storage temp.−20°C

Safety

Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Faceshields, Gloves
Hazard CodesXn
Risk Statements36/37/38-42
Safety Statements22-24-26-36/37
WGK Germany1

Related Products

SubstrateF5135, N-[3-(2-Furyl)acryloyl]-Leu-Gly-Pro-Ala
 C0627, Z-Gly-Pro-Leu-Gly-Pro
InhibitorM3531, (2R)-2-Mercaptomethyl-4-methylpentanoyl-β-(2-naphthyl)-Ala-Ala Amide

References

MerckMerck 13,2505